Coding

Part:BBa_I712003:Design

Designed by: Andrej Ondracka   Group: iGEM07_Ljubljana   (2007-10-19)

CCR5-NUb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 502
    Illegal XhoI site found at 1181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 183


Design Notes

PstI and EcoRI restriction sites inside CCR5 gene were mutated without changing the aminoacid sequence. CCR5 and NUb fragments were fused with PCR overlap extension method, without any linker added. The part is cloned into BBa_J52017, a vector with eukaryontic terminator.


Source

CCR5 gene obtained from OriGene, catalog No. SC110858. NUb gene obtained from Dualsystems, plasmid pDL2-Alg5.

References